DNA Vectors

DNA molecules used to transfer foreign genetic material into another cell, where it can be replicated and/or expressed. Products include cloning kits, transfection kits, expression kits, assay kits, and a variety of sequenced plasmids.

646 – 660 of 1,511 results

646

ATCC 4T1-GFP-Luc2 (CRL-2539-GFP-LUC2) Mouse mammary tumor cell line - dual GFP and luc2 (luciferase) reporter, adherent

4T1-GFP-Luc2 is a dual-reporter mouse mammary carcinoma cell line derived from the 4T1 parental line. It constitutively expresses green fluorescent protein (GFP) and firefly luciferase (luc2), enabling both fluorescent and bioluminescent detection for in vitro imaging, functional assays, and in vivo tumor monitoring. Cells are adherent and maintained under puromycin selection for routine culture; remove selection prior to co-culture or specific experimental procedures.

Species: Mus musculus (mouse).
Tissue/disease: Mammary gland; models metastatic breast cancer (stage IV).
Cell type: Epithelial-like, adherent growth.
Markers expressed: GFP and firefly luciferase (luc2) for fluorescence and bioluminescence readouts.
Storage: Maintain cryopreserved vials in vapor-phase liquid nitrogen.
Applications: In vitro fluorescent assays, bioluminescence assays, and in vivo tumor imaging and drug-response studies.

Pricing & Availability

647

ATCC HMC3 NFKB-LUC2

For-profit Organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Applications. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item. Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.

Pricing & Availability

648

BIOHIPPO AAV-D1-DIO-taCasp3-P2A-mCherry-WPREs

Recombinant adeno associated virus expressing taCasp3 fused to mCherry under the D1 promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.

Pricing & Availability

649

IBA LifeSciences pASK-IBA6C

pASK-IBA6C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tagII fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, and the sequence for a protease cleavage site.Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. The N-terminal Strep-tagII can be removed by the protease factor Xa.

Pricing & Availability

650

IBA LifeSciences StrGate Acptr Vctr pASG-IBAwt1

The pASG-IBAwt1 vector is designed for expression of target proteins without affinity tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.